近日，真邁生物自主研發(fā)的創(chuàng)新技術(shù)再次獲得國際認(rèn)可，包括全內(nèi)反射熒光成像系統(tǒng)在內(nèi)的三項(xiàng)發(fā)明專利正式通過歐洲專利局（European Patent Office）的授權(quán)批準(zhǔn)，成為有效歐洲專利。

至此，歷經(jīng)多年自主研發(fā)積累，真邁生物目前擁有的國內(nèi)外專利（含授權(quán)有效和申請中）及計算機(jī)軟件著作權(quán)登記等已近200件。

01

METHOD FOR CONTROLLING BASE SEQUENCE DETERMINATION?REACTION,?BASE SEQUENCE DETERMINATION SYSTEM AND CONTROL DEVICE

（對序列測定反應(yīng)進(jìn)行控制的方法、序列測定系統(tǒng)和控制裝置）

技術(shù)簡介：
序列測定系統(tǒng)包括流體裝置和光學(xué)裝置，反應(yīng)裝置包括第一單元和第二單元，定義序列測定反應(yīng)包含的一種重復(fù)執(zhí)行單位為第二生化反應(yīng)-第一生化反應(yīng)-拍照，方法包括在完成初始步驟之后，使得當(dāng)利用流體裝置使第一單元和第二單元中的一個上的樣品進(jìn)行第二生化反應(yīng)和第一生化反應(yīng)的同時，利用光學(xué)裝置對另一個單元上的樣品進(jìn)行拍照。初始步驟包括：a）利用流體裝置使第一單元和第二單元中的一個進(jìn)行樣品的第一生化反應(yīng)，b）利用光學(xué)裝置對進(jìn)行第一生化反應(yīng)后的單元的樣品的進(jìn)行拍照，c）利用流體裝置使第一單元和第二單元的另一個進(jìn)行樣品的第一生化反應(yīng)。上述方法可提高序列測定效率。
“The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c. using the fluid device to perform the first biochemical reaction of the sample on the second component. The above-mentioned method can improve the efficiency of base sequence determination.”

02

SINGLE-MOLECULE IMAGE CORRECTION METHOD, DEVICE AND?SYSTEM, AND COMPUTER-READABLE STORAGE MEDIUM

（單分子圖像糾偏方法及裝置）

?

技術(shù)簡介：
本專利公開了一種單分子圖像糾偏方法及裝置，其中方法包括：根據(jù)第一圖像的每一像素點(diǎn)對應(yīng)的亮度值矩陣，獲取與所述第一圖像對應(yīng)的第一目標(biāo)簡化矩陣，并根據(jù)第二圖像的每一像素點(diǎn)對應(yīng)的亮度值矩陣，獲取與所述第二圖像對應(yīng)的第二目標(biāo)簡化矩陣；對所述第一目標(biāo)簡化矩陣和所述第二目標(biāo)簡化矩陣分別進(jìn)行二維離散傅里葉變換，得到與所述第一目標(biāo)簡化矩陣對應(yīng)的第一傅里葉矩陣，并得到與所述第二目標(biāo)簡化矩陣對應(yīng)的第二傅里葉矩陣；根據(jù)所述第一傅里葉矩陣和所述第二傅里葉矩陣獲取所述第二圖像相對于所述第一圖像的偏移量；根據(jù)所述偏移量對所述第二圖像進(jìn)行糾偏?？梢?，實(shí)施本發(fā)明能夠?qū)Σ煌瑫r刻拍攝的單分子圖片進(jìn)行糾偏。
“An embodiment of the present disclosure discloses a method and a device of correcting a single-molecule image, the method includes: acquiring the first target simplified matrix corresponding to the first image according to the intensity matrix corresponding to each of pixels in the first image, and acquiring the second target simplified matrix corresponding to the second image according to the intensity matrix corresponding to each of the pixels in the second image; the two-dimensional DFT is performed to the first target simplified matrix and the second target simplified matrix respectively to acquire the first Fourier matrix corresponding to the first target simplified matrix and the second Fourier matrix corresponding to the second target simplified matrix; the method of correcting a single-molecule image acquire the offset of the second image with respect to the first image according to the first Fourier matrix and the second Fourier matrix; and correcting the second image according to the offset. Such method can correct the images of single molecules taken at different moments.”

03

TOTAL INTERNAL REFLECTION FLUORESCENCE IMAGING SYSTEMAND?SEQUENCING DEVICE

（全內(nèi)反射熒光成像系統(tǒng)及測序裝置）

技術(shù)簡介：

本專利公開了一種全內(nèi)反射熒光成像系統(tǒng)及測序裝置，全內(nèi)反射熒光成像系統(tǒng)包括第一成像系統(tǒng)和第二成像系統(tǒng)，所述第一成像系統(tǒng)包括N條獨(dú)立的激光光路，所述第二成像系統(tǒng)通過第二接收裝置獲取到第二像的位置的不同判斷樣品高度的變化，根據(jù)樣品高度的變化進(jìn)行自動補(bǔ)償，以保證樣品始終處于聚焦的位置。本發(fā)明可通過第二成像系統(tǒng)分別對N條獨(dú)立的激光光路進(jìn)行光路調(diào)節(jié)，以實(shí)現(xiàn)N條獨(dú)立的激光光路具有相同的穿透深度，進(jìn)而無需在切換不同光源時，再進(jìn)行光路調(diào)節(jié)，從而節(jié)省了實(shí)驗(yàn)當(dāng)中費(fèi)時的光路調(diào)節(jié)。

“The present disclosure discloses a total internal reflection fluorescence imaging system and a sequencing device. The total internal reflection fluorescence imaging system includes a first imaging system and a second imaging system. The first imaging system includes N separate laser light paths. The second imaging system determines the changes of the height of the sample by means of the differences of the positions at which the second receiving device acquires the second images, and performs automatic compensation according to the changes of the height of the sample, so as to ensure that the sample is always at a focused position. The embodiment can perform light path adjustment of the N separate laser light paths respectively by means of the second imaging system, so as to realize that the N separate laser light paths have the same penetration depth. Therefore no further light path adjustment is required when switching between different light sources, thus saving the time consuming for light path adjustment.”

自主研發(fā)、科技創(chuàng)新是真邁創(chuàng)立之初的內(nèi)在基因，也是真邁持續(xù)發(fā)展至今的戰(zhàn)略根基。為了有效支撐公司科技創(chuàng)新戰(zhàn)略，包括有效地對無形資產(chǎn)進(jìn)行轉(zhuǎn)化和管理運(yùn)營，公司一直秉承尊重科學(xué)家和工程師的團(tuán)隊(duì)文化，并據(jù)自身情況設(shè)立專門部門和人員進(jìn)行知識產(chǎn)權(quán)管理，公司知識產(chǎn)權(quán)的管理運(yùn)行符合國家推薦性標(biāo)準(zhǔn)GB/T 29490-2013《企業(yè)知識產(chǎn)權(quán)管理規(guī)范》。未來，真邁將進(jìn)一步加強(qiáng)深化知識產(chǎn)權(quán)布局，持續(xù)促進(jìn)公司核心競爭力的穩(wěn)步提升。